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Ligand binding assays quantify the amount of ERs with unoccupied ligand binding domains those that have not bound estrogen or a closely related molecule by measuring the amount of radiolabeled specific binding of estradiol in tissue homogenates 6 lasix contraindications This allows the patient to be reassured that he she will not die alone or in pain 14, 19, 28 and will assist the family in coping with their grief Figure 2
